Engineering Threshold-Based Selection Systems

Pedone, K. H.; Gonzalez-Perez, V.; Leopold, L. E.; Der, C. J.; Cox, A. D.; Ahmed, S.; Reiner, D. J.

doi:10.1101/2021.02.04.429784

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bioRxiv · 10.1101/2021.02.04.429784

Engineering Threshold-Based Selection Systems

Pedone, K. H.·Gonzalez-Perez, V.·Leopold, L. E.·Der, C. J.·Cox, A. D.·Ahmed, S.·Reiner, D. J.

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Abstract

Using model organisms to identify novel therapeutic targets is frequently constrained by pre-existing genetic toolkits. To expedite positive selection for identification of novel downstream effectors, we engineered conditional expression of activated CED-10/Rac to disrupt C. elegans embryonic morphogenesis, titrated to 100% lethality. The strategy of engineering thresholds for positive selection using experimental animals was validated with pharmacological and genetic suppression and is generalizable to diverse molecular processes and experimental systems.

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Pedone, K. H., Gonzalez-Perez, V., Leopold, L. E., Der, C. J., Cox, A. D., Ahmed, S., Reiner, D. J.. 2021-02-06. Engineering Threshold-Based Selection Systems. https://doi.org/10.1101/2021.02.04.429784

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