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bioRxiv · 10.1101/2021.02.02.429315

Perturbations in 3D genome organization can promote acquired drug resistance

Abstract

Acquired drug resistance is a major problem in the treatment of cancer. hTERT-immortalized, untransformed RPE-1 (RPE) cells can acquire resistance to taxol by derepressing the ABCB1 gene, encoding for the multidrug transporter P-gP. Here we have investigated how the ABCB1 gene is derepressed. We show that activation of the ABCB1 gene is associated with reduced DNA methylation, reduced H3K9 trimethylation and increased H3K27 acetylation at the ABCB1 promoter. In addition, we find that the ABCB1 locus has moved away from the nuclear lamina in the taxol-resistant cells. This raises the question which of these alterations were causal to derepression. Directly modifying DNA methylation or H3K27 methylation had neither significant effect on ABCB1 expression, nor did it promote drug resistance. In contrast, the disruption of Lamin B Receptor (LBR), a component of the nuclear lamina involved in genome organization, did promote the acquisition of a taxol-resistant phenotype in a subset of cells. Using CRISPRa-mediated gene activation, we could further substantiate a model in which disruption of lamina association renders the ABCB1 gene permissive to derepression. Based on these data we propose a model in which nuclear lamina dissociation of a repressed gene allows for its activation, implying that deregulation of the 3D genome topology could play an important role in tumor evolution and the acquisition of drug resistance.

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BibTeXRIS

Manjon, A. G., Peric-Hupkes, D., Liu, N. Q., Friskes, A., Joosten, S., Teunissen, H., Aarts, M., Prekovic, S., Zwart, W., de Wit, E., van Steensel, B., Medema, R. H.. 2021-02-02. Perturbations in 3D genome organization can promote acquired drug resistance. https://doi.org/10.1101/2021.02.02.429315

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