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bioRxiv · 10.1101/2021.02.01.429283

A Thermostable, Flexible RNA Vaccine Delivery Platform for Pandemic Response

Gerhardt, A.·Voigt, E.·Archer, M.·Reed, S.·Larson, E.·Van Hoeven, N.·Kramer, R.·Fox, C.·Casper, C.
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Abstract

Current RNA vaccines against SARS-CoV-2 are limited by instability of both the RNA and the lipid nanoparticle delivery system, requiring storage at -20{degrees}C or -70{degrees}C and compromising universally accessible vaccine distribution. This study demonstrates the thermostability and adaptability of a nanostructured lipid carrier (NLC) RNA vaccine delivery system for use in pandemic preparedness and pandemic response. Liquid NLC is stable at refrigerated temperatures for [≥] 1 year, enabling stockpiling and rapid deployment by point-of-care mixing with any vaccine RNA. Alternatively, NLC complexed with RNA may be readily lyophilized and stored at room temperature for [≥] 8 months or refrigerated temperature for [≥] 21 months. This thermostable RNA vaccine platform could significantly improve distribution of current and future pandemic response vaccines, particularly in low-resource settings. One Sentence SummaryAn RNA vaccine delivery system stable at room temperature for 8+ months and refrigerated for 21+ months.

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Gerhardt, A., Voigt, E., Archer, M., Reed, S., Larson, E., Van Hoeven, N., Kramer, R., Fox, C., Casper, C.. 2021-02-02. A Thermostable, Flexible RNA Vaccine Delivery Platform for Pandemic Response. https://doi.org/10.1101/2021.02.01.429283

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