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bioRxiv · 10.1101/2021.01.28.427517

Altered cleavage of Caspase-1 in hepatocytes limits control of malaria in the liver

Abstract

Malaria, caused by Plasmodium parasites, is a devastating disease that kills over half a million people each year1. Plasmodium sporozoites inoculated by mosquitoes into mammalian hosts undergo a clinically silent phase of obligatory development and replication in hepatocytes before initiating the life-threatening blood-stage of malaria2. Thus, understanding the immune responses elicited by Plasmodium infection in the liver is key to controlling clinical malaria and transmission3,4. Here, we show that Plasmodium DNA can be detected by AIM2 (absent in melanoma 2) sensors in the infected hepatocytes, resulting in Caspase-1 activation and pyroptotic cell-death. However, Caspase-1 was observed to undergo only partial cleavage in hepatocytes, limiting pyroptosis, and the maturation of pro-inflammatory cytokines classically associated with Caspase-1 activation. We discovered that the extent of Caspase-1 cleavage in cells is determined by the expression of ASC (apoptosis-associated speck-like protein containing a CARD). ASC expression is inherently low in hepatocytes, and transgenically enhancing it in the hepatocytes induced complete processing of Caspase-1, efficient secretion of pro-inflammatory cytokines, enhanced pyroptotic cell-death, and markedly improved control of malaria infection in the liver. In addition to describing a novel pathway of natural immunity to malaria, our findings uncover a key aspect of liver biology that may have been exploited during evolution by successful hepatotropic pathogens.

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BibTeXRIS

Marques-da-Silva, C., Poudel, B., Baptista, R. P., Peissig, K., Hancox, L. S., Shiau, J. C., Pewe, L., Shears, M. J., Kanneganti, T.-D., Sinnis, P., Kyle, D. E., Gurung, P., Harty, J. T., Kurup, S. P.. 2021-01-28. Altered cleavage of Caspase-1 in hepatocytes limits control of malaria in the liver. https://doi.org/10.1101/2021.01.28.427517

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