Genomic insights of high-risk clones of ESBL-producing Escherichia coli isolated from community infections and commercial meat in Southern Brazil

Soncini, J. G.; Cerdeira, L.; Koga, V. L.; Tizura, A. T.; Fuga, B.; Nakazato, G.; Kobayashi, R.; Aires, C. A. M.; Lincopan, N.; Vespero, E. C.

doi:10.1101/2020.12.31.424884

bioRxiv · 10.1101/2020.12.31.424884

Genomic insights of high-risk clones of ESBL-producing Escherichia coli isolated from community infections and commercial meat in Southern Brazil

Soncini, J. G.·Cerdeira, L.·Koga, V. L.·Tizura, A. T.·Fuga, B.·Nakazato, G.·Kobayashi, R.·Aires, C. A. M.·Lincopan, N.·Vespero, E. C.

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Abstract

During a microbiological and genomic surveillance study to investigate the molecular epidemiology of extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli from community-acquired urinary tract infections (UTI) and commercial meat samples, in a Brazilian city with a high occurrence of infections by ESBL-producing bacteria, we have identified the presence of CTX-M (-55, -27, -24, -15, -14 and -2)-producing E. coli belonging to the international clones ST354, ST131, ST117, and ST38. The ST131 was more prevalent in human samples, and worryingly the high-risk ST131-C1-M27 was identified in human infections for the first time. We also detected CTX-M-55-producing E. coli ST117 isolates from meat samples (i.e., chicken and pork) and human infections. Moreover, we have identified the important clone CTX-M-24-positive E. coli ST354 from human samples in Brazil for the first time. In brief, our results suggest a potential of commercialized meat as a reservoir of high-priority E. coli lineages in the community. In contrast, the identification of E. coli ST131-C1-M27 indicates that novel pandemic clones have emerged in Brazil, constituting a public health issue.

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Soncini, J. G., Cerdeira, L., Koga, V. L., Tizura, A. T., Fuga, B., Nakazato, G., Kobayashi, R., Aires, C. A. M., Lincopan, N., Vespero, E. C.. 2021-01-02. Genomic insights of high-risk clones of ESBL-producing Escherichia coli isolated from community infections and commercial meat in Southern Brazil. https://doi.org/10.1101/2020.12.31.424884

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