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bioRxiv · 10.1101/2020.12.24.424341

Metaproteomic Profiling of Fungal Gut Colonization in Gnotobiotic Mice

Pettersen, V. K.·Dufour, A.·Arrieta, M. C.
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Abstract

Eukaryotic microbes can modulate mammalian host health and disease states, yet the molecular contribution of gut fungi remains nascent. We previously showed that mice exclusively colonized with fungi displayed increased sensitivity to allergic airway inflammation and fecal metabolite profiles similar to germ-free mice. To gain insights into the functional changes attributed to fungal colonization, we performed quantitative proteomic analyses of feces and small intestine of four-week-old gnotobiotic mice colonized with bacteria, fungi, or both. A comparison of fecal metaproteomic profiles between the mouse groups yielded broad changes in the relative levels of bacterial (46% of 2,860) and mouse (76% from 405) proteins. Many of the detected fungal proteins (3% of 1,492) have been previously reported as part of extracellular vesicles and having immunomodulating properties. Changes in the levels of mouse proteins derived from the jejunum (4% of 1,514) were mainly driven by proteins functional in lipid metabolism and apoptosis. Using metaproteomic profiling of gnotobiotic conditions, we show that fungal colonization profoundly impacts the host gut proteome. Our results suggest that an increased abundance of certain gut fungal species in early life may impact the developing intracellular balance of epithelial and immune cells.

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Pettersen, V. K., Dufour, A., Arrieta, M. C.. 2020-12-24. Metaproteomic Profiling of Fungal Gut Colonization in Gnotobiotic Mice. https://doi.org/10.1101/2020.12.24.424341

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