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bioRxiv · 10.1101/2020.12.21.423810

Pathogen effector recognition-dependent association of NRG1 with EDS1 and SAG101 in TNL receptor immunity

Sun, X.·Lapin, D.·Feehan, J. M.·Stolze, S. C.·Kramer, K.·Dongus, J. A.·Rzemieniewski, J.·Blanvillain-Baufume, S.·Harzen, A.·Bautor, J.·Derbyshire, P.·Menke, F. L. H.·Finkemeier, I.·Nakagami, H.·Jones, J. D. G.·Parker, J. E.
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Abstract

Plants utilise intracellular nucleotide-binding, leucine-rich repeat (NLR) immune receptors to detect pathogen effectors and activate local and systemic defence. NRG1 and ADR1 "helper" NLRs (RNLs), cooperate with enhanced disease susceptibility 1 (EDS1), senescence-associated gene 101 (SAG101) and phytoalexin-deficient 4 (PAD4) lipase-like proteins to mediate signalling from TIR domain NLR receptors (TNLs). However, the mechanism of RNL/EDS1-family protein cooperation is poorly understood. Here, we provide genetic and molecular evidence for exclusive EDS1/SAG101/NRG1 and EDS1/PAD4/ADR1 co-functions in TNL immunity. Using immunoprecipitation and mass spectrometry, we show effector recognition-dependent association of NRG1 with EDS1 and SAG101, but not PAD4. An EDS1-SAG101 complex associates with NRG1, and EDS1-PAD4 associates with ADR1, only in an immune-activated state. NRG1 requires an intact nucleotide-binding P-loop motif, and EDS1 a functional EP domain and its partner SAG101, for induced association and immunity. Thus, two distinct modules (NRG1/EDS1/SAG101 and ADR1/EDS1/PAD4) are required to execute TNL receptor defence signalling.

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Sun, X., Lapin, D., Feehan, J. M., Stolze, S. C., Kramer, K., Dongus, J. A., Rzemieniewski, J., Blanvillain-Baufume, S., Harzen, A., Bautor, J., Derbyshire, P., Menke, F. L. H., Finkemeier, I., Nakagami, H., Jones, J. D. G., Parker, J. E.. 2020-12-22. Pathogen effector recognition-dependent association of NRG1 with EDS1 and SAG101 in TNL receptor immunity. https://doi.org/10.1101/2020.12.21.423810

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