Do initial concentration and activated sludge seasonality affect pharmaceutical biodegradation rate constants?

van Bergen, T. J. H. M.; Rios-Miguel, A. B.; Nolte, T. M.; Ragas, A. M.; van Zelm, R.; Graumans, M. H.; Scheepers, P. T.; Jetten, M. S.; Hendriks, A. J.; Welte, C. U.

doi:10.1101/2020.12.17.423224

bioRxiv · 10.1101/2020.12.17.423224

Do initial concentration and activated sludge seasonality affect pharmaceutical biodegradation rate constants?

van Bergen, T. J. H. M.·Rios-Miguel, A. B.·Nolte, T. M.·Ragas, A. M.·van Zelm, R.·Graumans, M. H.·Scheepers, P. T.·Jetten, M. S.·Hendriks, A. J.·Welte, C. U.

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Abstract

Pharmaceuticals find their way to the aquatic environment via wastewater treatment plants (WWTPs) and biodegradation plays an important role in mitigating environmental risks, however a mechanistic understanding of involved processes is limited. The aim of this study was to evaluate potential relationships between first-order biodegradation rate constants (kb) of nine pharmaceuticals and initial concentration of the selected compounds, and sampling season of the used activated sludge inocula. Four-day bottle experiments were performed with activated sludge from WWTP Groesbeek (The Netherlands) of two different seasons, summer and winter, spiked with two environmentally relevant concentrations (3 and 30 nM) of pharmaceuticals. Concentrations of the compounds were measured by LC-MS/MS, microbial community composition was assessed by 16S rRNA gene amplicon sequencing and kb values were calculated. The biodegradable pharmaceuticals, ranked from high to low biodegradation rates, were acetaminophen, metformin, metoprolol, terbutaline, and phenazone. Carbamazepine, diatrizoic acid, diclofenac and fluoxetine were not converted. Summer and winter inocula did not show significant differences in microbial community composition, but resulted in a slightly different kb for some pharmaceuticals. Likely microbial activity was responsible instead of community composition. In the same inoculum different kb values were measured, depending on initial concentration. In general, biodegradable compounds had a higher kb when the initial concentration was higher. This demonstrates that Michealis-Menten kinetics theory has shortcomings for some pharmaceuticals at low, environmentally relevant concentrations and that the pharmaceutical concentration should be taken into account when measuring the kb in order to reliably predict the fate of pharmaceuticals in the WWTP.

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van Bergen, T. J. H. M., Rios-Miguel, A. B., Nolte, T. M., Ragas, A. M., van Zelm, R., Graumans, M. H., Scheepers, P. T., Jetten, M. S., Hendriks, A. J., Welte, C. U.. 2020-12-17. Do initial concentration and activated sludge seasonality affect pharmaceutical biodegradation rate constants?. https://doi.org/10.1101/2020.12.17.423224

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