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bioRxiv · 10.1101/2020.12.12.422522

Conformational Shannon entropy of mRNA structures from force spectroscopy measurements predicts the efficiency of -1 programmed ribosomal frameshift stimulation

Halma, M. T.·Ritchie, D. B.·Woodside, M. T.
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Abstract

-1 Programmed ribosomal frameshifting (-1 PRF) is stimulated by structures in mRNA, but the factors determining -1 PRF efficiency are unclear. We show that -1 PRF efficiency varies directly with the conformational heterogeneity of the stimulatory structure, quantified as the Shannon entropy of the state occupancy, for a panel of stimulatory structures with efficiency ranging from 2-80%. The correlation is force-dependent and vanishes at forces above those applied by the ribosome. This work supports the hypothesis that heterogeneous conformational dynamics are a key factor in stimulating -1 PRF.

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Halma, M. T., Ritchie, D. B., Woodside, M. T.. 2020-12-13. Conformational Shannon entropy of mRNA structures from force spectroscopy measurements predicts the efficiency of -1 programmed ribosomal frameshift stimulation. https://doi.org/10.1101/2020.12.12.422522

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