The genomic epidemiology of multi-drug resistant nontyphoidal Salmonella causing invasive disease in sub-Saharan Africa

Park, S. E.; Pham, D. T.; Pak, G. D.; Panzner, U.; Espinoza, L. M. C.; von Kalckreuth, V.; Im, J.; Mogeni, O. D.; Schutt-Gerowitt, H.; Crump, J. A.; BREIMAN, R. F.; Abu-Sarkodie, Y.; Owusu-Dabo, E.; Rakotozandrindrainy, R.; Soura, A. B.; Aseffa, A.; Gasmelseed, N.; Sooka, A.; Keddy, K. H.; May, J.; Aaby, P.; Biggs, H. M.; Hertz, J. T.; Montgomery, J. M.; Cosmas, L.; Olack, B.; Fields, B.; Sarpong, N.; Razafindrabe, T. J. L.; Raminosoa, T. M.; Kabore, L. P.; Sampo, E.; Teferi, M.; Yeshitela, B.; Tayeb, M. A. E.; Krumkamp, R.; Dekker, D. M.; Jaeger, A.; Tall, A.; Niang, A.; Bjerregaard-Andersen,

doi:10.1101/2020.12.11.422246

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bioRxiv · 10.1101/2020.12.11.422246

The genomic epidemiology of multi-drug resistant nontyphoidal Salmonella causing invasive disease in sub-Saharan Africa

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Abstract

BackgroundInvasive nontyphoidal Salmonella (iNTS) is one of the leading causes of bacteraemia in sub-Saharan Africa. Multi-drug resistance (MDR) and further resistance to third generation cephalosporins and fluoroquinolones have emerged in multiple iNTS serotypes. Molecular epidemiological investigations of nontyphoidal Salmonella are needed to better understand the genetic characteristics and transmission dynamics associated with major MDR iNTS serotypes across the continent. MethodsA total of 166 nontyphoidal Salmonella isolates causing invasive disease were collected from a multi-centre study in eight African countries between 2010 and 2014, and whole-genome sequenced to investigate the geographical distribution, antimicrobial genetic determinants and population structure of iNTS serotypes-genotypes. Phylogeographical reconstruction was further conducted in context of the existing genomic framework of iNTS serotypes Typhimurium and Enteritidis. Population-based incidence of MDR-iNTS disease was also estimated. ResultsSalmonella enterica subsp. Enterica serotype Typhimurium (S. Typhimurium) sequence-type (ST) 313 and Salmonella enterica subsp. Enterica serotype Enteritidis (S. Enteritidis) ST11 were predominant, and both exhibited high frequencies of MDR. Salmonella enterica subsp. Enterica serotype Dublin (S. Dublin) ST10 emerged in West Africa. Mutations in the gyrA gene were identified in S. Enteritidis and S. Typhimurium in Ghana; and ST313 carrying blaCTX-M-15 was found in Kenya. Inter-country transmission of MDR ST313 lineage II and the West African Clade of MDR ST11 between Ghana and neighbouring countries including Mali, Burkina Faso, and Nigeria were evident. The incidence of MDR-iNTS disease exceeded 100/100,000-person years-of-observation (PYO) in children aged <5 years in several West African countries. ConclusionsMultiple MDR iNTS serotypes-sequence types, predominantly S. Typhimurium ST313 and S. Enteritidis ST11, are co-circulating in sub-Saharan Africa with evidence of transmission between West African countries. The development of safe and effective iNTS vaccines coupled with appropriate antimicrobial stewardship and adequate epidemiological monitoring are essential to limit the impact of these pathogens in Africa.

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Park, S. E., Pham, D. T., Pak, G. D., Panzner, U., Espinoza, L. M. C., von Kalckreuth, V., Im, J., Mogeni, O. D., Schutt-Gerowitt, H., Crump, J. A., BREIMAN, R. F., Abu-Sarkodie, Y., Owusu-Dabo, E., Rakotozandrindrainy, R., Soura, A. B., Aseffa, A., Gasmelseed, N., Sooka, A., Keddy, K. H., May, J., Aaby, P., Biggs, H. M., Hertz, J. T., Montgomery, J. M., Cosmas, L., Olack, B., Fields, B., Sarpong, N., Razafindrabe, T. J. L., Raminosoa, T. M., Kabore, L. P., Sampo, E., Teferi, M., Yeshitela, B., Tayeb, M. A. E., Krumkamp, R., Dekker, D. M., Jaeger, A., Tall, A., Niang, A., Bjerregaard-Andersen,. 2020-12-12. The genomic epidemiology of multi-drug resistant nontyphoidal Salmonella causing invasive disease in sub-Saharan Africa. https://doi.org/10.1101/2020.12.11.422246

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