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bioRxiv · 10.1101/2020.12.07.413112

Structural basis for piRNA-targeting

Anzelon, T. A.·Chowdhury, S.·Hughes, S. M.·Xiao, Y.·Lander, G. C.·MacRae, I. J.
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Abstract

Piwi proteins use PIWI-interacting RNAs (piRNAs) to identify and silence the transposable elements (TEs) pervasively found in animal genomes. The Piwi targeting mechanism is proposed to be similar to targeting by Argonaute proteins, which employ microRNA (miRNA) guides to repress cellular mRNAs, but has not been characterized in detail. We present cryo-EM structures of a Piwi-piRNA complex with and without target RNAs and analysis of target recognition. Resembling Argonaute, Piwi identifies targets using the piRNA seed-region. However, Piwi creates a much weaker seed so that prolonged target association requires further piRNA-target pairing. Beyond the seed, Piwi creates wide central cleft wide for unencumbered piRNA-target pairing, enabling long-lived Piwi-piRNA-target interactions that are tolerant of mismatches. Piwi ensures targeting fidelity by blocking propagation of the piRNA-target duplex in the absence of faithful seed pairing, and by requiring extended piRNA-target pairing to reach an endonucleolytically active conformation. This mechanism allows Piwi to minimize off-targeting cellular mRNAs and adapt piRNA sequences to evolving genomic threats.

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Anzelon, T. A., Chowdhury, S., Hughes, S. M., Xiao, Y., Lander, G. C., MacRae, I. J.. 2020-12-07. Structural basis for piRNA-targeting. https://doi.org/10.1101/2020.12.07.413112

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