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bioRxiv · 10.1101/2020.12.02.408120

Single-cell intracellular epitope and transcript detection revealing signal transduction dynamics

Rivello, F.·van Buijtenen, E.·Matula, K.·van Buggenum, J.·Vink, P.·van Eenennaam, H.·Mulder, K. W.·Huck, W. T. S.
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Abstract

Current high-throughput single-cell multi-omics methods cannot concurrently map changes in (phospho)protein levels and the associated gene expression profiles. We present QuRIE-seq (Quantification of RNA and Intracellular Epitopes by sequencing) and use multi-factor omics analysis (MOFA+) to map signal transduction over multiple timescales. We demonstrate that QuRIE-seq can trace the activation of the B-cell receptor pathway at the minute and hour time-scale and provide insight into the mechanism of action of an inhibitory drug, Ibrutinib.

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Rivello, F., van Buijtenen, E., Matula, K., van Buggenum, J., Vink, P., van Eenennaam, H., Mulder, K. W., Huck, W. T. S.. 2020-12-02. Single-cell intracellular epitope and transcript detection revealing signal transduction dynamics. https://doi.org/10.1101/2020.12.02.408120

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