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bioRxiv · 10.1101/2020.11.25.397968

An adaptive optics module for deep tissue multiphoton imaging in vivo

Rodriguez, C.·Chen, A.·Rivera, J. A.·Mohr, M. A.·Liang, Y.·Sun, W.·Milkie, D. E.·Bifano, T. G.·Chen, X.·Ji, N.
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Abstract

Understanding complex biological systems requires visualizing structures and processes deep within living organisms. We developed a compact adaptive optics module and incorporated it into two- and three-photon fluorescence microscopes, to measure and correct tissue-induced aberrations. We resolved synaptic structures in deep cortical and subcortical areas of the mouse brain, and demonstrated high-resolution imaging of neuronal structures and somatosensory-evoked calcium responses in the mouse spinal cord at unprecedented depths in vivo.

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Rodriguez, C., Chen, A., Rivera, J. A., Mohr, M. A., Liang, Y., Sun, W., Milkie, D. E., Bifano, T. G., Chen, X., Ji, N.. 2020-11-26. An adaptive optics module for deep tissue multiphoton imaging in vivo. https://doi.org/10.1101/2020.11.25.397968

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