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bioRxiv · 10.1101/2020.11.09.373282

Full species-wide leaf and seed ionomic diversity of Arabidopsis thaliana

Campos, A. C. A. L.·van Dijk, W. F. A.·Ramakrishna, P.·Giles, T.·Korte, P.·Douglas, A.·Smith, P.·Salt, D. E.
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Abstract

O_LISoil is a heterogenous reservoir of essential elements needed for plant growth and development. Plants have evolved mechanisms to balance their nutritional needs based on availability of nutrients. This has led to genetically-based variation in the elemental composition ionome, of plants, both within and between species. C_LIO_LIWe explore this natural variation using a panel of wild-collected, geographically widespread Arabidopsis thaliana accessions from the 1001 Genomes Project including over 1,135 accessions, and the 19 parental accessions of the Multi-parent Advanced Generation Inter-Cross (MAGIC) panel, all with full-genome sequences available. C_LIO_LIWe present an experimental design pipeline for high-throughput ionomic screenings and analyses with improved normalisation procedures to account for errors and variability in conditions often encountered in large-scale, high-throughput data collection. We report quantification of the complete leaf and seed ionome of the entire collection using this pipeline and a digital tool-IonExplorer to interact with the dataset. C_LIO_LIWe describe the pattern of natural ionomic variation across the A. thaliana species and identify several accessions with extreme ionomic profiles. It forms a valuable resource for exploratory QTL, GWA studies to identify genes underlying natural variation in leaf and seed ionome and genetic adaptation of plants to soil conditions. C_LI

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Campos, A. C. A. L., van Dijk, W. F. A., Ramakrishna, P., Giles, T., Korte, P., Douglas, A., Smith, P., Salt, D. E.. 2020-11-10. Full species-wide leaf and seed ionomic diversity of Arabidopsis thaliana. https://doi.org/10.1101/2020.11.09.373282

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