Two-component spike nanoparticle vaccine protects macaques from SARS-CoV-2 infection

Brouwer, P. J. M.; Brinkkemper, M.; Maisonnasse, P.; Dereuddre-Bosquet, N.; Grobben, M.; Claireaux, M.; de Gast, M.; Marlin, R.; Chesnais, V.; Diry, S.; Allen, J. D.; Watanabe, Y.; Griezen, J. M.; Kerster, G.; Turner, H. L.; van der Straten, K.; van der Linden, C. A.; Aldon, Y.; Naninck, T.; Bontjer, I.; Burger, J. A.; Poniman, M.; Mykytyn, A. Z.; Okba, N. M. A.; Schermer, E. E.; van Breemen, M. J.; Ravichandran, R.; Caniels, T. G.; van Schooten, J.; Kahlaoui, N.; Contreras, V.; Lemaitre, J.; Chapon, C.; Ho Tsong Fang, R.; Villaudy, J.; Sliepen, K.; van der Velden, Y. U.; Haagmans, B.; de Bree

doi:10.1101/2020.11.07.365726

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bioRxiv · 10.1101/2020.11.07.365726

Two-component spike nanoparticle vaccine protects macaques from SARS-CoV-2 infection

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Abstract

The SARS-CoV-2 pandemic is continuing to disrupt personal lives, global healthcare systems and economies. Hence, there is an urgent need for a vaccine that prevents viral infection, transmission and disease. Here, we present a two-component protein-based nanoparticle vaccine that displays multiple copies of the SARS-CoV-2 spike protein. Immunization studies show that this vaccine induces potent neutralizing antibody responses in mice, rabbits and cynomolgus macaques. The vaccine-induced immunity protected macaques against a high dose challenge, resulting in strongly reduced viral infection and replication in upper and lower airways. These nanoparticles are a promising vaccine candidate to curtail the SARS-CoV-2 pandemic.

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Brouwer, P. J. M., Brinkkemper, M., Maisonnasse, P., Dereuddre-Bosquet, N., Grobben, M., Claireaux, M., de Gast, M., Marlin, R., Chesnais, V., Diry, S., Allen, J. D., Watanabe, Y., Griezen, J. M., Kerster, G., Turner, H. L., van der Straten, K., van der Linden, C. A., Aldon, Y., Naninck, T., Bontjer, I., Burger, J. A., Poniman, M., Mykytyn, A. Z., Okba, N. M. A., Schermer, E. E., van Breemen, M. J., Ravichandran, R., Caniels, T. G., van Schooten, J., Kahlaoui, N., Contreras, V., Lemaitre, J., Chapon, C., Ho Tsong Fang, R., Villaudy, J., Sliepen, K., van der Velden, Y. U., Haagmans, B., de Bree. 2020-11-08. Two-component spike nanoparticle vaccine protects macaques from SARS-CoV-2 infection. https://doi.org/10.1101/2020.11.07.365726

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