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bioRxiv · 10.1101/2020.11.03.367086

Analysing the Yeast Complexome - The Complex Portal rising to the challenge

Meldal, B. H. M.·Pons, C.·Perfetto, L.·del-Toro, N.·Wong, E.·Aloy, P.·Hermjakob, H.·Orchard, S.·Porras, P.
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Abstract

The EMBL-EBI Complex Portal is a knowledgebase of macromolecular complexes providing persistent stable identifiers. Entries are linked to literature evidence and provide details of complex membership, function, structure and complex-specific Gene Ontology annotations. Data is freely available and downloadable in HUPO-PSI community standards and missing entries can be requested for curation. In collaboration with Saccharomyces Genome Database and UniProt, the yeast complexome, a compendium of all known heteromeric assemblies from the model organism Saccharomyces cerevisiae, was curated. This expansion of knowledge and scope has led to a 50% increase in curated complexes compared to the previously published dataset, CYC2008. The yeast complexome is used as a reference resource for the analysis of complexes from large-scale experiments. Our analysis showed that genes coding for proteins in complexes tend to have more genetic interactions, are co-expressed with more genes, are multifunctional, localize more often in the nucleus, and are more often involved in nucleic acid-related metabolic processes and processes where large machineries are the predominant functional drivers. A comparison to genetic interactions showed that about 40% of expanded co-complex pairs also have genetic interactions, suggesting strong functional links between complex members.

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Meldal, B. H. M., Pons, C., Perfetto, L., del-Toro, N., Wong, E., Aloy, P., Hermjakob, H., Orchard, S., Porras, P.. 2020-11-04. Analysing the Yeast Complexome - The Complex Portal rising to the challenge. https://doi.org/10.1101/2020.11.03.367086

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