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bioRxiv · 10.1101/2020.11.02.364497

The SARS-CoV-2 RNA interactome

Lee, S.·Lee, Y.-s.·Choi, Y.·Son, A.·Park, Y.·Lee, K.-M.·Kim, J.·Kim, J.-S.·Kim, V. N.
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Abstract

SARS-CoV-2 is an RNA virus whose success as a pathogen relies on its ability to repurpose host RNA-binding proteins (RBPs) to form its own RNA interactome. Here, we developed and applied a robust ribonucleoprotein capture protocol to uncover the SARS-CoV-2 RNA interactome. We report 109 host factors that directly bind to SARS-CoV-2 RNAs including general antiviral factors such as ZC3HAV1, TRIM25, and PARP12. Applying RNP capture on another coronavirus HCoV-OC43 revealed evolutionarily conserved interactions between viral RNAs and host proteins. Network and transcriptome analyses delineated antiviral RBPs stimulated by JAK-STAT signaling and proviral RBPs responsible for hijacking multiple steps of the mRNA life cycle. By knockdown experiments, we further found that these viral-RNA-interacting RBPs act against or in favor of SARS-CoV-2. Overall, this study provides a comprehensive list of RBPs regulating coronaviral replication and opens new avenues for therapeutic interventions.

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Lee, S., Lee, Y.-s., Choi, Y., Son, A., Park, Y., Lee, K.-M., Kim, J., Kim, J.-S., Kim, V. N.. 2020-11-02. The SARS-CoV-2 RNA interactome. https://doi.org/10.1101/2020.11.02.364497

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