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bioRxiv · 10.1101/2020.10.26.355073

Atomic-level evolutionary information improves protein-protein interface scoring

Quignot, C.·Granger, P.·Chacon, P.·Guerois, R.·Andreani, J.
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Abstract

The crucial role of protein interactions and the difficulty in characterising them experimentally strongly motivates the development of computational approaches for structural prediction. Even when protein-protein docking samples correct models, current scoring functions struggle to discriminate them from incorrect decoys. The previous incorporation of conservation and coevolution information has shown promise for improving protein-protein scoring. Here, we present a novel strategy to integrate atomic-level evolutionary information into different types of scoring functions to improve their docking discrimination. We applied this general strategy to our residue-level statistical potential from InterEvScore and to two atomic-level scores, SOAP-PP and Rosetta interface score (ISC). Including evolutionary information from as few as ten homologous sequences improves the top 10 success rates of these individual scores by respectively 6.5, 6 and 13.5 percentage points, on a large benchmark of 752 docking cases. The best individual homology-enriched score reaches a top 10 success rate of 34.4%. A consensus approach based on the complementarity between different homology-enriched scores further increases the top 10 success rate to 40%. All data used for benchmarking and scoring results, as well as pipelining scripts, are available at http://biodev.cea.fr/interevol/interevdata/

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Quignot, C., Granger, P., Chacon, P., Guerois, R., Andreani, J.. 2020-10-26. Atomic-level evolutionary information improves protein-protein interface scoring. https://doi.org/10.1101/2020.10.26.355073

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