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bioRxiv · 10.1101/2020.10.22.349720

In silico secretome characterization of clinical Mycobacterium abscessus isolates provides insights into antigenic differences

Cornejo-Granados, F.·Kohl, T. A.·Sotomayor, F. V.·Andres, S.·Hernandez-Pando, R.·Hurtado-Ramirez, J. M.·Utpatel, C.·Niemann, S.·Maurer, F. P.·Ochoa-Leyva, A.
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Abstract

Mycobacterium abscessus (MAB) is a widely disseminated pathogenic non-tuberculous mycobacterium (NTM). Like with M. tuberculosis complex (MTBC), excreted / secreted (ES) proteins play an essential role for its virulence and survival inside the host. ES proteins contain highly immunogenic proteins, which are of interest for novel diagnostic assays and vaccines. Here, we used a robust bioinformatics pipeline to predict the secretome of the M. abscessus ATCC 19977 reference strain and fifteen clinical isolates belonging to all three MAB subspecies, M. abscessus subsp. abscessus, M. abscessus subsp. bolletii, and M. abscessus subsp. massiliense. We found that ~18% of the proteins encoded in the MAB genomes were predicted as secreted and that the three MAB subspecies shared > 85 % of the predicted secretomes. MAB isolates with a rough (R) colony morphotype showed larger predicted secretomes than isolates with a smooth (S) morphotype. Additionally, proteins exclusive to the secretomes of MAB R variants had higher antigenic densities than those exclusive to S variants, independently of the subspecies. For all investigated isolates, ES proteins had a significantly higher antigenic density than non-ES proteins. We identified 337 MAB ES proteins with homologues in previously investigated M. tuberculosis secretomes. Among these, 222 have previous experimental support of secretion, and some proteins showed homology with protein drug targets reported in the DrugBank database. The predicted MAB secretomes showed a higher abundance of proteins related to quorum-sensing and Mce domains as compared to MTBC indicating the importance of these pathways for MAB pathogenicity and virulence. Comparison of the predicted secretome of M. abscessus ATCC 19977 with the list of essential genes revealed that 99 secreted proteins corresponded to essential proteins required for in vitro growth. All predicted secretomes were deposited in the Secret-AAR web-server (http://microbiomics.ibt.unam.mx/tools/aar/index.php).

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Cornejo-Granados, F., Kohl, T. A., Sotomayor, F. V., Andres, S., Hernandez-Pando, R., Hurtado-Ramirez, J. M., Utpatel, C., Niemann, S., Maurer, F. P., Ochoa-Leyva, A.. 2020-10-22. In silico secretome characterization of clinical Mycobacterium abscessus isolates provides insights into antigenic differences. https://doi.org/10.1101/2020.10.22.349720

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