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bioRxiv · 10.1101/2020.10.13.337733

High-resolution architecture of human epiphysis formation

Sun, H.·Wen, Y.·Wu, W.·Qin, T.·An, C.·Fan, C.·Chen, Y.·Ji, J.·Chew, T. G.·Chen, J.·Ouyang, H.
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Abstract

Human limb skeletal system consists of both bone and cartilage which originated from fetal cartilage. However, the roadmap of chondrocyte divergent differentiation to bone and articular cartilage has yet to be established. Epiphysis possesses articular cartilage, growth plate and the secondary ossification center (SOC), making it an ideal model to uncover the trajectory of chondrocyte divergent differentiation. Here, we mapped differentiation trajectory of human chondrocyte during postnatal finger epiphysis development by using single-cell RNA sequencing. Our results uncovered that chondroprogenitors have two differentiation pathways to hypertrophic chondrocytes during ossification, and one pathway to articular chondrocytes for formation of cartilages. Interestingly, we found that, as an addition to the known typical endochondral ossification path from resting, proliferative to hypertrophic chondrocytes, there was a bypass by which chondroprogenitors differentiate into hypertrophic chondrocytes without proliferative stage. Furthermore, our results revealed two new chondrocyte subpopulations (bypass chondrocytes as it appeared in the ossification bypass, and ID1+ chondroblasts in articular chondrocyte path) during postnatal epiphysis development in addition to six well-known subpopulations. Overall, our study provides a comprehensive roadmap of chondrocyte differentiation in human epiphysis thereby expanding the knowledge of bone and articular cartilage, which could be utilized to design biotherapeutics for bone and articular cartilage regeneration.

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Sun, H., Wen, Y., Wu, W., Qin, T., An, C., Fan, C., Chen, Y., Ji, J., Chew, T. G., Chen, J., Ouyang, H.. 2020-10-14. High-resolution architecture of human epiphysis formation. https://doi.org/10.1101/2020.10.13.337733

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