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bioRxiv · 10.1101/2020.10.05.327122

Lysophosphatidic acid (LPA)-antibody (504B3)validation by free-solution assay and interferometry identifies off-target binding

Ray, M.·Kihara, Y.·Bornhop, D. J.·Chun, J.
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Abstract

Antibody specificity and sensitivity is required in basic and clinical research for ensuring scientific rigor and reproducibility, while off-target cross-reactivity could generate erroneous conclusions. Lysophosphatidic acid (LPA) is a bioactive lipid being targeted clinically by antibody strategies. Here, we reexamined binding properties of a commercially available monoclonal antibody (504B3) reported as specific for LPA using a free-solution assay measured in a compensated interferometric reader. The antibody showed comparable binding affinities to LPA and non-LPA lipids including phosphatidic acid (PA) and lysophosphatidylcholine (LPC). These results may alter conclusions drawn from current and past basic and clinical studies employing anti-LPA antibodies.

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Ray, M., Kihara, Y., Bornhop, D. J., Chun, J.. 2020-10-05. Lysophosphatidic acid (LPA)-antibody (504B3)validation by free-solution assay and interferometry identifies off-target binding. https://doi.org/10.1101/2020.10.05.327122

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