SunRISE: long-term imaging of individual mRNA molecules in living cells

Guo, Y.; Lee, R. E. C.

doi:10.1101/2020.10.05.326660

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bioRxiv · 10.1101/2020.10.05.326660

SunRISE: long-term imaging of individual mRNA molecules in living cells

Guo, Y.·Lee, R. E. C.

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Abstract

Single-cell imaging of individual mRNAs has revealed core mechanisms of the central dogma. However, most approaches require cell fixation or have limited sensitivity for live-cell applications. Here, we describe SunRISE (SunTag-based Reporter for Imaging Signal Enriched mRNA), a computationally and experimentally optimized approach for unambiguous single-mRNA detection in living cells. We demonstrate SunRISE with long-term epifluorescence imaging, using translational stress to track mRNA phase separation and recovery from cytosolic droplets.

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Guo, Y., Lee, R. E. C.. 2020-10-06. SunRISE: long-term imaging of individual mRNA molecules in living cells. https://doi.org/10.1101/2020.10.05.326660

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