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bioRxiv · 10.1101/2020.09.30.320887

PyHDX: Derivation and visualization of protection factors from Hydrogen-Deuterium Exchange Mass Spectrometry at near residue resolution

Smit, J. H.·Krishnamurthy, S.·Srinivasu, B. Y.·Karamanou, S.·Economou, A.
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Abstract

Hydrogen Deuterium Exchange Mass Spectrometry is a powerful monitor of protein intrinsic dynamics, yet the interpretation, visualization and cross-comparison of HDX-MS datasets is challenging. Here we present PyHDX, an open-source python package and web server, that batch-extracts the universal quantity Gibbs free energy at residue level over multiple protein conditions and homologues. {Delta}G values relate to protein normal modes and together provide a universal measure of protein flexibility. AvailabilityPyHDX source code is released under the MIT license and can be accessed on GitHub.

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Smit, J. H., Krishnamurthy, S., Srinivasu, B. Y., Karamanou, S., Economou, A.. 2020-10-02. PyHDX: Derivation and visualization of protection factors from Hydrogen-Deuterium Exchange Mass Spectrometry at near residue resolution. https://doi.org/10.1101/2020.09.30.320887

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