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bioRxiv · 10.1101/2020.09.29.318378

The antimicrobial peptide TAT-RasGAP317-326 inhibits the formation and the expansion of bacterial biofilms in vitro

Heinonen, T.·Hargraves, S.·Georgieva, M.·Widmann, C.·Jacquier, N.
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Abstract

Biofilms are structured aggregates of bacteria embedded in a self-produced matrix. Pathogenic bacteria can form biofilms on surfaces and in tissues leading to nosocomial and chronic infections. While antibiotics are largely inefficient in limiting biofilm formation and expansion, antimicrobial peptides (AMPs) are emerging as alternative anti-biofilm treatments. In this study, we explore the effect of the newly described AMP TAT-RasGAP317-326 on Acinetobacter baumannii, Pseudomonas aeruginosa and Staphylococcus aureus biofilms. We observe that TAT-RasGAP317-326 inhibits the formation of biofilms at concentrations equivalent or two times superior to the minimal inhibitory concentration (MIC) of the corresponding planktonic bacteria. Moreover, TAT-RasGAP317-326 limits the expansion of A. baumannii and P. aeruginosa established biofilms at concentrations 2-4 times superior to the MIC. These results further confirm the potential of AMPs against biofilms, expand the antimicrobial potential of TAT-RasGAP317-326 and support further development of this peptide as an alternative antimicrobial treatment.

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Heinonen, T., Hargraves, S., Georgieva, M., Widmann, C., Jacquier, N.. 2020-09-29. The antimicrobial peptide TAT-RasGAP317-326 inhibits the formation and the expansion of bacterial biofilms in vitro. https://doi.org/10.1101/2020.09.29.318378

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