Carbon for nutrient exchange between the lycophyte, Lycopodiella inundata and Mucoromycotina fine root endophytes is unresponsive to high atmospheric CO2 concentration

Hoysted, G. A.; Kowal, J. A.; Pressel, S.; Duckett, J. G.; Bidartondo, M. I.; Field, K. J.

doi:10.1101/2020.09.29.318022

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bioRxiv · 10.1101/2020.09.29.318022

Carbon for nutrient exchange between the lycophyte, Lycopodiella inundata and Mucoromycotina fine root endophytes is unresponsive to high atmospheric CO2 concentration

Hoysted, G. A.·Kowal, J. A.·Pressel, S.·Duckett, J. G.·Bidartondo, M. I.·Field, K. J.

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Abstract

Background and AimsNon-vascular plants associating with arbuscular mycorrhizal (AMF) and Mucoromycotina fine root endophyte (MFRE) fungi derive greater benefits from their fungal associates under higher atmospheric [CO2] than ambient, however nothing is known about how changes in [CO2] affects MFRE function in vascular plants. MethodsWe measured movement of phosphorus (P), nitrogen (N) and carbon (C) between the lycophyte, Lycopodiella inundata and Mucoromycotina fine root endophyte fungi using 33P-orthophosphate, 15N-ammonium chloride and 14CO2 isotope tracers under ambient and elevated atmospheric [CO2] concentrations of 440 and 800 ppm, respectively. Key ResultsTransfer of 33P and 15N from MFRE to plant were unaffected by changes in [CO2]. There was a slight increase in C transfer from plant to MFRE under elevated [CO2]. ConclusionsOur results demonstrate that the exchange of C-for-nutrients between a vascular plant and Mucoromycotina FRE is largely unaffected by changes in atmospheric [CO2]. Unravelling the role of MFRE in host plant nutrition and potential C-for-N trade changes between symbionts under varying abiotic conditions is imperative to further our understanding of the past, present and future roles of diverse plant-fungal symbioses in global ecosystems.

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Hoysted, G. A., Kowal, J. A., Pressel, S., Duckett, J. G., Bidartondo, M. I., Field, K. J.. 2020-10-01. Carbon for nutrient exchange between the lycophyte, Lycopodiella inundata and Mucoromycotina fine root endophytes is unresponsive to high atmospheric CO2 concentration. https://doi.org/10.1101/2020.09.29.318022

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