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bioRxiv · 10.1101/2020.09.27.313262

DIMT1, a regulator of ribosomal biogenesis, controls beta-cell protein synthesis, mitochondrial function and insulin secretion

Abstract

We previously reported that transcription factor B1 mitochondrial (TFB1M) is involved in the pathogenesis of type 2 diabetes (T2D) owing to mitochondrial dysfunction. Here, we describe that dimethyladenosine transferase 1 homolog (DIMT1), a homologue of TFB1M, is expressed and active in pancreatic {beta}-cells. Like TFB1M, it has been implicated in control of ribosomal RNA (rRNA) but its role in {beta}-cells or T2D remains to be identified. Silencing of DIMT1 impacted mitochondrial function, leading to reduced expression of mitochondrial OXPHOS proteins, reduced oxygen consumption rate (OCR), dissipated mitochondrial membrane potential ({Delta}{Psi}m) and caused a lower rate of ATP production (mATP). In addition, DIMT1 knockdown slowed the rate of protein synthesis. In accordance with these findings, DIMT1-deficiency perturbed insulin secretion in rodent and human {beta}-cell lines. These effects are likely a result of destabilization of ribosomal assembly, involving NIN1 (RPN12) binding protein 1 homolog (NOB-1) and Pescadillo ribosomal biogenesis factor 1 (PES-1). These are two critical ribosomal subunits proteins, whose interactions were perturbed upon DIMT1-deficiency, thereby disturbing protein synthesis in {beta}-cells. Thus, we have here highlighted a role of DIMT1 in ribosomal biogenesis that perturbs protein synthesis, resulting in mitochondrial dysfunction and disrupted insulin secretion, both being potential pathogenetic factors in T2D.

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BibTeXRIS

Mulder, H., Verma, G., Bowen, A., Hamilton, A., Esguerra, J., Karagiannopoulos, A., Buscunan, R. C., Lyons, C., Cowan, E., Fex, M.. 2020-09-27. DIMT1, a regulator of ribosomal biogenesis, controls beta-cell protein synthesis, mitochondrial function and insulin secretion. https://doi.org/10.1101/2020.09.27.313262

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