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bioRxiv · 10.1101/2020.09.20.305508

Diesel2p mesoscope with dual independent scan engines for flexible capture of dynamics in distributed neural circuitry

Yu, C.-H.·Stirman, J. N.·Yu, Y.·Hira, R.·Smith, S. L.
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Abstract

Imaging the activity of neurons that are widely distributed across brain regions deep in scattering tissue at high speed remains challenging. Here, we introduce an open-source system with Dual Independent Enhanced Scan Engines for Large Field-of-view Two-Photon imaging (Diesel2p). Combining novel optical design, adaptive optics, and temporal multiplexing, the system offers subcellular resolution over a large field-of-view ([~] 25 mm2) with independent scan engines. We demonstrate the flexibility and various use cases of this system for calcium imaging of neurons in the living brain.

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Yu, C.-H., Stirman, J. N., Yu, Y., Hira, R., Smith, S. L.. 2020-09-20. Diesel2p mesoscope with dual independent scan engines for flexible capture of dynamics in distributed neural circuitry. https://doi.org/10.1101/2020.09.20.305508

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