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bioRxiv · 10.1101/2020.09.14.297440

Fate of the M-phase-assembled centrioles during the cell cycle in the TP53;PCNT;CEP215-deleted cells

Jung, G. I.·Rhee, K.
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Abstract

Cancer cells frequently include supernumerary centrioles. Here, we generated TP53;PCNT;CEP215 triple knockout cell lines and observed precocious separation and amplification of the centrioles at M phase. Many of the triple KO cells maintained supernumerary centrioles throughout the cell cycle. The M-phase-assembled centrioles lack an ability to function as templates for centriole assembly during S phase. They also lack an ability to organize microtubules in interphase. However, we found that a fraction of them acquired an ability to organize microtubules during M phase. Our works provide an example how supernumerary centrioles behave in dividing cells.

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Jung, G. I., Rhee, K.. 2020-09-15. Fate of the M-phase-assembled centrioles during the cell cycle in the TP53;PCNT;CEP215-deleted cells. https://doi.org/10.1101/2020.09.14.297440

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