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bioRxiv · 10.1101/2020.09.11.293993

SOX9 is sufficient to drive endothelial cells towards a mesenchymal fate by altering the chromatin landscape.

Fuglerud, B. M.·Drissler, S.·Stephan, T. L.·Thakur, A.·Cullum, R.·Hoodless, P. A.
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Abstract

The transcription factor SOX9 is expressed in multiple tissues during embryogenesis and directs developmental processes. SOX9 is activated upon endothelial-to-mesenchymal transition (EndMT) in the developing heart, but its role in regulating this process is less clear. Using human umbilical vein endothelial cells as an EndMT model, we show that SOX9 expression alone is sufficient to activate mesenchymal enhancers and steer endothelial cells towards a mesenchymal fate. By genome-wide mapping of the chromatin landscape, we show that SOX9 acts as a pioneer transcription factor, having the ability to open chromatin and lead to deposition of active histone marks at a specific subset of previously silent enhancers, guided by SOX motifs and H2A.Z enrichment. This leads to a switch in enhancer activity states resulting in activation of mesenchymal genes and concurrent suppression of endothelial genes to drive EndMT. Moreover, we show that SOX9 chromatin binding is dynamic, but induces stable changes in the chromatin landscape. Our data also show widespread SOX9 chromatin scanning in silent chromatin that is not associated with SOX motifs or H2A.Z enrichment. Our study highlights the crucial developmental role of SOX9 and provides new insight into key molecular functions of SOX9 in the chromatin landscape and mechanisms of EndMT. Graphical abstract O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=94 SRC="FIGDIR/small/293993v2_ufig1.gif" ALT="Figure 1"> View larger version (20K): org.highwire.dtl.DTLVardef@1248576org.highwire.dtl.DTLVardef@b6420forg.highwire.dtl.DTLVardef@1aaffc5org.highwire.dtl.DTLVardef@17de585_HPS_FORMAT_FIGEXP M_FIG C_FIG

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Fuglerud, B. M., Drissler, S., Stephan, T. L., Thakur, A., Cullum, R., Hoodless, P. A.. 2020-09-11. SOX9 is sufficient to drive endothelial cells towards a mesenchymal fate by altering the chromatin landscape.. https://doi.org/10.1101/2020.09.11.293993

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