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bioRxiv · 10.1101/2020.09.11.293225

Acquisition of Host Cytosolic Protein by Toxoplasma gondii Bradyzoites

Kannan, G.·Thaprawat, P.·Schultz, T. L.·Carruthers, V. B.
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Abstract

Toxoplasma gondii is a protozoan parasite that persists in the central nervous system as intracellular chronic stage bradyzoites that are encapsulated by a thick cyst wall. While the cyst wall separates bradyzoites from the host cytosol, it has been posited that small solutes can traverse the cyst wall to sustain bradyzoites. Recently it was found that host cytosolic macromolecules can cross the parasitophorous vacuole and are ingested and digested by actively replicating acute stage tachyzoites. However, the extent to which bradyzoites have an active ingestion pathway remained unknown. To interrogate this, we modified previously published protocols that look at tachyzoite acquisition and digestion of host proteins by measuring parasite accumulation of a host-expressed reporter protein after impairment of an endolysosomal protease (Cathepsin Protease L, CPL). Using two cystogenic parasite strains (ME49 and Pru), we demonstrate that T. gondii bradyzoites can ingest host-derived cytosolic mCherry. Bradyzoites acquire host mCherry within 4 hours of invasion and post-cyst wall formation. This study provides direct evidence that host macromolecules can be internalized by T. gondii bradyzoites across the cyst wall in infected cells.

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Kannan, G., Thaprawat, P., Schultz, T. L., Carruthers, V. B.. 2020-09-11. Acquisition of Host Cytosolic Protein by Toxoplasma gondii Bradyzoites. https://doi.org/10.1101/2020.09.11.293225

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