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bioRxiv · 10.1101/2020.09.10.288878

The chromosomal passenger complex establishes chromosome biorientation via two parallel localization pathways

Abstract

Chromosome biorientation is established by the four-member chromosomal passenger complex (CPC) through phosphorylation of incorrect kinetochore-microtubule attachments. During chromosome alignment, the CPC localizes to the inner centromere, the inner kinetochore and spindle microtubules. Here we show that a small region of the CPC subunit INCENP/Sli15 is required to target the complex to all three of these locations in budding yeast. This region, the SAH, is essential for phosphorylation of outer kinetochore substrates, chromosome segregation, and viability. By restoring the CPC to each of these three locations individually, we found that inner centromere localization is sufficient to establish chromosome biorientation and viability independently of the other two targeting mechanisms. Remarkably, although neither the inner kinetochore nor microtubule binding activities was able to rescue viability individually, they were able to do so when combined. We have therefore identified two parallel pathways by which the CPC can promote chromosome biorientation and proper completion of mitosis.

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BibTeXRIS

Marsoner, T., Yedavalli, P., Masnovo, C., Schmitzer, K., Campbell, C.. 2020-09-11. The chromosomal passenger complex establishes chromosome biorientation via two parallel localization pathways. https://doi.org/10.1101/2020.09.10.288878

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