Identification and characterization of distinct murine brown adipocyte lineages

Ussar, S.; Karlina, R.; Lutter, D.; Miok, V.; Fischer, D. S.; Altun, I.; Schoettl, T.; Schorpp, K.; Israel, A.; Cero, C.; Johnson, J. W.; Kapser-Fischer, I.; Boettcher, A.; Keipert, S.; Feuchtinger, A.; Graf, E.; Strom, T. M.; Walch, A.; Lickert, H.; Walzthoeni, T.; Heinig, M.; Theis, F. J.; Garcia-Caceres, C.; Cypess, A. M.

doi:10.1101/2020.08.24.264416

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bioRxiv · 10.1101/2020.08.24.264416

Identification and characterization of distinct murine brown adipocyte lineages

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Abstract

Brown adipose tissue (BAT) plays an important role in the regulation of body weight and glucose homeostasis. While increasing evidence supports white adipose tissue heterogeneity, little is known about heterogeneity within murine BAT. Using single cell RNA sequencing of the stromal vascular fraction of murine BAT and analysis of 67 brown preadipocyte and adipocyte clones we unravel heterogeneity within brown preadipocytes. Statistical analysis of gene expression profiles from these clones identifies markers distinguishing brown adipocyte lineages. We confirm the presence of distinct brown adipocyte populations in vivo using three identified markers; Eif5, Tcf25, and Bin1. Functionally, we demonstrate that loss of Bin1 enhances UCP1 expression and mitochondrial respiration, suggesting that Bin1 marks a dormant brown adipocyte type. The existence of multiple brown adipocyte lineages suggests distinct functional properties of BAT depending on its cellular composition, with potentially distinct function in thermogenesis and the regulation of whole body energy homeostasis.

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Ussar, S., Karlina, R., Lutter, D., Miok, V., Fischer, D. S., Altun, I., Schoettl, T., Schorpp, K., Israel, A., Cero, C., Johnson, J. W., Kapser-Fischer, I., Boettcher, A., Keipert, S., Feuchtinger, A., Graf, E., Strom, T. M., Walch, A., Lickert, H., Walzthoeni, T., Heinig, M., Theis, F. J., Garcia-Caceres, C., Cypess, A. M.. 2020-08-25. Identification and characterization of distinct murine brown adipocyte lineages. https://doi.org/10.1101/2020.08.24.264416

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