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bioRxiv · 10.1101/2020.08.24.263418

ELAVL1 Exclusively Couples mRNA Stability with the 3'UTRs of Interferon Stimulated Genes

Abstract

Upon detection of a pathogen, the innate immune system triggers signaling events leading to the transcription of mRNAs that encode for pro-inflammatory and anti-microbial effectors. RNA-binding proteins (RBPs) interact with these functionally critical mRNAs and temporally regulate their fates at the post-transcriptional level. One such RBP is ELAVL1, which is known to bind to introns and 3UTRs. While significant progress has been made in understanding how ELAVL1 regulates mRNAs, how its target repertoire and binding affinity changes within an immunological context remains poorly understood. Here, we overlap four distinct high-throughput approaches to define its cell-type and context-dependent targets and determine its regulatory impact during immune activation. ELAVL1 overwhelmingly binds to intronic sites in a naive state, but during an innate immune response, ELAVL1 targets the 3UTR - binding both previously and newly expressed mRNAs. We find that ELAVL1 mediates the RNA stability of genes that regulate the pathways involved in pathogen sensing and cytokine production. Our findings reveal the importance of examining RBP regulatory impact under dynamic transcriptomic events to best understand their post-transcriptional regulatory roles within specific biological circuitries.

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BibTeXRIS

Rothamel, K. L., Arcos, S. E., Kim, B., Reasoner, C., Mukherjee, N., Ascano, M.. 2020-08-24. ELAVL1 Exclusively Couples mRNA Stability with the 3'UTRs of Interferon Stimulated Genes. https://doi.org/10.1101/2020.08.24.263418

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