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bioRxiv · 10.1101/2020.08.18.255877

Drug Repurposing Screen for Compounds Inhibiting the Cytopathic Effect of SARS-CoV-2

Chen, C. Z.·Shinn, P.·Itkin, Z.·Eastman, R.·Bostwick, R.·Rasmussen, L.·Huang, R.·Shen, M.·Hu, X.·Wilson, K. M.·Brooks, B.·Guo, H.·Zhao, T.·Klumpp-Thomas, C.·Simeonov, A.·Michael, S. G.·Lo, D. C.·Hall, M.·Zheng, W.
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Abstract

Drug repurposing is a rapid approach to identifying therapeutics for the treatment of emerging infectious diseases such as COVID-19. To address the urgent need for treatment options, we carried out a quantitative high-throughput screen using a SARS-CoV-2 cytopathic assay with a compound collection of 8,810 approved and investigational drugs, mechanism-based bioactive compounds, and natural products. Three hundred and nineteen compounds with anti-SARS-CoV-2 activities were identified and confirmed, including 91 approved drug and 49 investigational drugs. Among these confirmed compounds, the anti-SARS-CoV-2 activities of 230 compounds, including 38 approved drugs, have not been previously reported. Chlorprothixene, methotrimeprazine, and piperacetazine were the three most potent FDA approved drugs with anti-SARS-CoV-2 activities. These three compounds have not been previously reported to have anti-SARS-CoV-2 activities, although their antiviral activities against SARS-CoV and Ebola virus have been reported. These results demonstrate that this comprehensive data set of drug repurposing screen for SARS-CoV-2 is useful for drug repurposing efforts including design of new drug combinations for clinical trials.

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Chen, C. Z., Shinn, P., Itkin, Z., Eastman, R., Bostwick, R., Rasmussen, L., Huang, R., Shen, M., Hu, X., Wilson, K. M., Brooks, B., Guo, H., Zhao, T., Klumpp-Thomas, C., Simeonov, A., Michael, S. G., Lo, D. C., Hall, M., Zheng, W.. 2020-08-18. Drug Repurposing Screen for Compounds Inhibiting the Cytopathic Effect of SARS-CoV-2. https://doi.org/10.1101/2020.08.18.255877

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