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bioRxiv · 10.1101/2020.08.17.254771

A High-Performance Genetically Encoded Fluorescent Biosensor for Imaging Physiological Peroxynitrite

Chen, Z.·Zhang, S.·Li, X.·Ai, H.
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Abstract

Peroxynitrite is a highly reactive nitrogen species (RNS) that plays critical roles in signal transduction, stress response, and numerous human diseases. Advanced molecular tools that permit the selective, sensitive, and non-invasive detection of peroxynitrite is essential for understanding its pathophysiological functions. Here, we present pnGFP-Ultra, a high performance, reaction-based, genetically encodable biosensor for imaging peroxynitrite in live cells. pnGFP-Ultra features a p-boronophenylalanine-modified chromophore as the sensing moiety and exhibits a remarkable 123-fold fluorescence turn-on response towards peroxynitrite while displaying virtually no cross-reaction with other reactive oxygen/nitrogen species, including hydrogen peroxide. To facilitate the expression of pnGFP-Ultra in mammalian cells, we engineered a highly efficient noncanonical amino acid (ncAA) expression system that is broadly applicable to the mammalian expression of proteins containing various ncAAs. pnGFP-Ultra robustly detected peroxynitrite production during interferon {gamma} and lipopolysaccharide-induced immune responses in macrophages, and in amyloid {beta}-activated primary glial cells. Thus, pnGFP-Ultra fills an important technical gap and represents an important new addition to the molecular toolbox in probing RNS biology. In BriefChen et al. report pnGFP-Ultra, a high-performance fluorescent biosensor for minimally invasive and selective imaging of peroxynitrite production in live cells. HighlightsO_LIpnGFP-Ultra is a genetically encoded peroxynitrite biosensor with a 123-fold fluorescence turn-on response C_LIO_LIpnGFP-Ultra exhibits high selectivity toward peroxynitrite, with virtually no crossreaction with hydrogen peroxide C_LIO_LIAn optimized plasmid-based system increases noncanonical amino acid incorporation in mammalian cells by >10 fold C_LIO_LIpnGFP-Ultra robustly detects peroxynitrite production in macrophages and primary glial cells C_LI

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Chen, Z., Zhang, S., Li, X., Ai, H.. 2020-08-18. A High-Performance Genetically Encoded Fluorescent Biosensor for Imaging Physiological Peroxynitrite. https://doi.org/10.1101/2020.08.17.254771

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