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bioRxiv · 10.1101/2020.08.17.254359

Role of selective Bioactive Compounds as an Angiotensin Converting Enzyme Inhibitor

Khan, H.·Hussain, T.·Kataria, M.·SETH, A.·Malik, M. Z.·Dash, A.·Chand, S.·KHAN, M. A.
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Abstract

Hypertension is one of a major reason of mortality and morbidity and it is associated with heart and renal disease. The aim of this study is to find out the antihypertensive role of bioactive compounds from selected medicinal plants targeting ACE molecule which so far is not known. The plants taken in this study were Moringa oleifera, Azadirachta indica, and Hibiscus sabdariffa. The nitric oxide and superoxide scavenging property vary from 39.50% to 68% and 37.67 % to 75.50 %. respectively. The inhibition of ACE activity was found maximally in methanolic extract of A. indica (74 %), followed by H. sabdariffa (73.4%), and least in M. oleifera (71.8 %). The bioactive chloroform fraction was characterized for the presence of compound using standard techniques such as LCMS and NMR (13C-NMR 1H-NMR). The results revealed the presence of beta-sitosterol in M. oleifera, azadiradionolide in A. indica and hibiscitrin in H. sabdariffa. The compounds have shown significant low binding energy for hibiscitrin (-12.3kcal/mol), beta-sitosterol (-11.2kcal/mol) and azadiradionolide (-11.3kcal/mol) indicating the high efficacy of binding on the enzyme. While, binding energy of drug captopril was -5.6kcal/mol & enalpril - 8.1kcal/mol in the same pocket of the ACE molecule. Upon subjecting molecular dynamic simulation results indicated that beta sitosterol complex provided more compactness than the hibiscitrin and azadiradionolide compounds. The current study delivers a new perspective for the drug development against systolic blood pressure regulation and also opens new horizons for considering alternate highly potent drug target for hypertension.

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Khan, H., Hussain, T., Kataria, M., SETH, A., Malik, M. Z., Dash, A., Chand, S., KHAN, M. A.. 2020-08-19. Role of selective Bioactive Compounds as an Angiotensin Converting Enzyme Inhibitor. https://doi.org/10.1101/2020.08.17.254359

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