Response of mycorrhizae to herbivory and soil moisture in a semiarid grazing ecosystem

Abstract

Arbuscular mycorrhizal fungal (AMF) symbioses with plants can be influenced by top-down forces such as grazing, and also by bottom-up forces such as soil resource availability, both of which are being altered by anthropogenic and global change drivers. While the influence of each of these factors on AMF symbioses has been widely studied, explicit tests of the relative strengths of top-down versus bottom-up influences on these ubiquitous plant root symbioses are few. We studied AMF colonization responses of four species of graminoids (3 grasses Elymus longae-aristatus, Leymus secalinus and Stipa orientalis, and a sedge Carex melanantha) common to semiarid high-altitude rangelands of the Spiti region, Trans-Himalaya, to changes in a top-down driver, grazing intensity (through short-term clipping and long-term grazer exclusion experiments), and a bottom-up driver, water availability (using irrigation treatments, and by evaluating responses to annual precipitation levels across years). Over three years, AMF colonization in all four host species was influenced by precipitation, with the highest and lowest AMF colonization levels corresponding to years with the lowest and highest rainfall, respectively. However, responses to long-term grazer exclusion differed among host species, and across years: while some species showed decreases in AMF colonization levels under grazing, others showed increases from ungrazed control levels, and these responses changed, even reversed, across years. Responses to short-term clipping and irrigation treatments also differed among hosts, with some species responding to irrigation alone, some to clipping and irrigation combined, and others showing no changes in AMF colonization from control levels in any of the treatments. In our study, long-term changes in water availability influenced AMF colonization levels, while short-term responses were host specific. Responses to above-ground tissue loss, however, differed among host species both in the long- and short-term. Overall, this study demonstrates that while AMF colonization levels correspond to annual precipitation levels in this semiarid ecosystem, host species also play a role in influencing plant-AMF interactions in these rangelands, with colonization levels and responses to abiotic factors changing with host species.