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bioRxiv · 10.1101/2020.07.31.231399

Structure of a type IV CRISPR-Cas effector complex

Zhou, Y.·Bravo, J. P. K.·Taylor, H. N.·Steens, J.·Jackson, R. N.·Staals, R. H. J.·Taylor, D. W.
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Abstract

We reveal the structure of a type IV-B CRISPR effector (Csf) complex at 3.9 [A] resolution using cryo-electron microscopy. The complex resembles the type III-A CRISPR Csm effector complex, but lacks subunits for RNA processing and target DNA cleavage, and is surprisingly assembled upon heterogeneous non-CRISPR RNA. These findings provide the first glimpse into the assembly and function of enigmatic type IV CRISPR systems.

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Zhou, Y., Bravo, J. P. K., Taylor, H. N., Steens, J., Jackson, R. N., Staals, R. H. J., Taylor, D. W.. 2020-07-31. Structure of a type IV CRISPR-Cas effector complex. https://doi.org/10.1101/2020.07.31.231399

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