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bioRxiv · 10.1101/2020.07.31.228486

SARS-CoV-2 protein subunit vaccination elicits potent neutralizing antibody responses

Mandolesi, M.·Sheward, D. J.·Hanke, L.·Ma, J.·Pushparaj, P.·Perez Vidakovics, L.·Kim, C.·Lore, K.·Castro Dopico, X.·Coquet, J. M.·McInerney, G.·Karlsson Hedestam, G. B.·Murrell, B.
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Abstract

The outbreak and spread of SARS-CoV-2 (Severe Acute Respiratory Syndrome coronavirus 2), the cause of coronavirus disease 2019 (COVID-19), is a current global health emergency and a prophylactic vaccine is needed urgently. The spike glycoprotein of SARS-CoV-2 mediates entry into host cells, and thus is a target for neutralizing antibodies and vaccine design. Here we show that adjuvanted protein immunization with SARS-CoV-2 spike trimers, stabilized in prefusion conformation 1, results in potent antibody responses in mice and rhesus macaques with neutralizing antibody titers orders of magnitude greater than those typically measured in serum from SARS-CoV-2 seropositive humans. Neutralizing antibody responses were observed after a single dose, with exceptionally high titers achieved after boosting. Furthermore, neutralizing antibody titers elicited by a dose-sparing regimen in mice were similar to those obtained from a high dose regimen. Taken together, these data strongly support the development of adjuvanted SARS-CoV-2 prefusion-stabilized spike protein subunit vaccines.

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Mandolesi, M., Sheward, D. J., Hanke, L., Ma, J., Pushparaj, P., Perez Vidakovics, L., Kim, C., Lore, K., Castro Dopico, X., Coquet, J. M., McInerney, G., Karlsson Hedestam, G. B., Murrell, B.. 2020-07-31. SARS-CoV-2 protein subunit vaccination elicits potent neutralizing antibody responses. https://doi.org/10.1101/2020.07.31.228486

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