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bioRxiv · 10.1101/2020.07.30.229997

Notch-Directed Germ Cell Proliferation Is Mediated by Proteoglycan-Dependent Transcription

Gopal, S.·Amran, A.·Elton, A.·Ng, L.·Pocock, R.
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Abstract

Notch receptors are essential membrane-bound regulators of cell proliferation and differentiation in metazoa. In the nematode Caenorhabditis elegans, correct expression of GLP-1 (germline proliferation-1), a germline-expressed Notch receptor, is important for germ cell maintenance. However, mechanisms that regulate GLP-1 expression are undefined. Here, we demonstrate that an AP-2 transcription factor (APTF-2) regulates GLP-1 expression through calcium-dependent binding to a conserved motif in the glp-1 promoter. Our data reveals that SDN-1 (syndecan-1), a transmembrane proteoglycan, regulates a TRP calcium channel in the soma to modulate the interaction between APTF-2 and glp-1 promoter - thus providing a potential communication nexus between the germline and its somatic environment to control germ cell fate decisions.

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Gopal, S., Amran, A., Elton, A., Ng, L., Pocock, R.. 2020-07-31. Notch-Directed Germ Cell Proliferation Is Mediated by Proteoglycan-Dependent Transcription. https://doi.org/10.1101/2020.07.30.229997

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