Resistance to tembotrione, a 4-hydroxyphenylpyruvate dioxygenase-inhibitor, in Sorghum bicolor

Pandian, B. A.; Varanasi, A.; Vennapusa, A. R.; Rajendran, S.; Lin, G.; Zhao, M.; Tunnell, M.; Tesso, T.; Liu, S.; Prasad, P. V. V.; Jugulam, M.

doi:10.1101/2020.07.29.227512

bioRxiv · 10.1101/2020.07.29.227512

Resistance to tembotrione, a 4-hydroxyphenylpyruvate dioxygenase-inhibitor, in Sorghum bicolor

Pandian, B. A.·Varanasi, A.·Vennapusa, A. R.·Rajendran, S.·Lin, G.·Zhao, M.·Tunnell, M.·Tesso, T.·Liu, S.·Prasad, P. V. V.·Jugulam, M.

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Abstract

Postemergence grass weed control continues to be a big challenge in grain sorghum (Sorghum bicolor L. Moench), primarily due to a lack of herbicide options registered for use in this crop. The development of herbicide-resistant sorghum technology to facilitate broad-spectrum postemergence weed control is an economical and viable solution. The 4-hydroxyphenylpyruvate dioxygenase-inhibitor herbicides (e.g. mesotrione or tembotrione) can control broad-spectrum of weeds including grasses, which, however, is not registered for postemergence application in sorghum due to crop injury. In this study we identified two tembotrione-resistant sorghum genotypes (G-200, G-350) and one highly susceptible genotype (S-1) through screening 317 sorghum lines from the sorghum association panel (SAP). Compared to S-1, G-200 and G-350 exhibited 10- and 7-fold more resistance to tembotrione, respectively. Genetic analyses of the F1 and F2 progeny generated from a cross between tembotrione-resistant and -susceptible genotypes demonstrated that the resistance is a semi-dominant polygenic trait. Furthermore, cytochrome P450 (CYP)-inhibitor assay using malathion and piperonyl butoxide suggested possible CYP-mediated metabolism of tembotrione in G-200 and G-350. Genotype-by-sequencing based quantitative trait loci (QTL) mapping revealed eight QTLs associated with tembotrione resistance in grain sorghum. Sorghum genotypes G-200 and G-350 confer a high level of metabolic resistance to tembotrione and controlled by a polygenic trait. There is an enormous potential to introgress the tembotrione resistance into breeding lines to develop agronomically desirable sorghum hybrids. One-sentence summaryThis research focuses on characterization, genetic analyses, identification of QTLs-linked to metabolic resistance to tembotrione in Sorghum bicolor, for improved weed control and increased yield

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Pandian, B. A., Varanasi, A., Vennapusa, A. R., Rajendran, S., Lin, G., Zhao, M., Tunnell, M., Tesso, T., Liu, S., Prasad, P. V. V., Jugulam, M.. 2020-07-30. Resistance to tembotrione, a 4-hydroxyphenylpyruvate dioxygenase-inhibitor, in Sorghum bicolor. https://doi.org/10.1101/2020.07.29.227512

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