Leptospira spp. seroprevalence in humans involved in a cross-sectional study in Garissa and Tana River Counties, Kenya

Mutembei, A.; Mutai, F. K.; Mwololo, D.; Muriuki, J.; Obonyo, M.; Kairu-Wanyoike, S. W.; Wainaina, M.; Lindahl, J.; Ontiri, E.; Bukachi, S.; Njeru, I.; Karanja, J.; Grace, D.; Bett, B.

doi:10.1101/2020.07.17.208363

bioRxiv · 10.1101/2020.07.17.208363

Leptospira spp. seroprevalence in humans involved in a cross-sectional study in Garissa and Tana River Counties, Kenya

Mutembei, A.·Mutai, F. K.·Mwololo, D.·Muriuki, J.·Obonyo, M.·Kairu-Wanyoike, S. W.·Wainaina, M.·Lindahl, J.·Ontiri, E.·Bukachi, S.·Njeru, I.·Karanja, J.·Grace, D.·Bett, B.

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Abstract

IntroductionLeptospirosis is a neglected bacterial zoonotic infection caused by spirochetes of Leptospira genus. Humans get infected through direct or indirect contact with urine of infected animals or environment. It accounts for more than 300,000 severe cases annually worldwide with case fatality rates of over 30%. Costs of diagnosis and treatment for human and animals, disruption of international trade of animals and products, reduced productivity and reproductivity in animals constitute economic importance. In Kenya, leptospirosis burden is significant but under-diagnosis and under-reporting affects the awareness of the disease. This study aimed to determine and compare the sero-prevalence and factors associated with Leptospira spp. in the two counties. MethodsWe conducted a cross-sectional study that involved apparently healthy people of at least 5 years of age in randomly selected households in Garissa and Tana River Counties. Blood samples were collected and tested for Leptospira spp antibodies using IgM ELISA. Standardized structured questionnaires were administered to collect socio-demographic and exposure information. We calculated frequencies and proportions for categorical variables and odds ratios (OR) and 95% confidence interval (CI) to evaluate association between sero-positivity and exposure factors. We used Wilcoxon test to evaluate statistical difference in sero-positivity for continuous variables and calculated test statistic (H) and p-value. ResultsA total of 952 subjects were recruited into the study - these included 482 persons from Garissa and 470 from Tana River. The overall sero-prevalence was 26% [(244/952); (CI: 23% to 29%)]. Garissa County had significantly higher Leptospira spp. seroprevalence (31%, n = 147; CI: 27% to 35%) compared to Tana River County (21 %, n = 97; CI: 17% to 25%). Being a female (OR=1.6, CI: 1.2-2.2) and engaging in pastoralism (OR=2.7, CI: 1.8-3.9) were significantly associated with higher odds of Leptospira spp. seropositivity compared to being a male or working in irrigated areas. The mean altitude of residence of sero-positive patients was 73m {+/-} 21 SD (standard deviation) above sea level and that for sero-negative was 80m {+/-} 22 SD (H=35, p-value = 0.00). ConclusionThis study determined the seroprevalence and risk factors for Leptospira spp. exposure in Garissa and Tana River Counties, Kenya. Females in pastoral communities experience high burden of the disease. Enhanced surveillance in humans and animals and further research is required to understand the complex and multifactorial drivers of leptospirosis transmission in the two Counties.

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Mutembei, A., Mutai, F. K., Mwololo, D., Muriuki, J., Obonyo, M., Kairu-Wanyoike, S. W., Wainaina, M., Lindahl, J., Ontiri, E., Bukachi, S., Njeru, I., Karanja, J., Grace, D., Bett, B.. 2020-07-17. Leptospira spp. seroprevalence in humans involved in a cross-sectional study in Garissa and Tana River Counties, Kenya. https://doi.org/10.1101/2020.07.17.208363

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