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bioRxiv · 10.1101/2020.07.10.196295

Metabolic features of mouse and human retinas: rods vs. cones, macula vs. periphery, retina vs. RPE

Bo Li·Ting Zhang·Wei Liu·Yekai Wang·Rong Xu·Shaoxue Zeng·Rui Zhang·Mark C Gillies·Ling Zhu·Jianhai Du
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Abstract

Photoreceptors, especially cones, which are enriched in the human macula, have high energy demands, making them vulnerable to metabolic stress. Metabolic dysfunction of photoreceptors and their supporting retinal pigment epithelium (RPE) is an important underlying cause of degenerative retinal diseases. However, how cones and the macula support their exorbitant metabolic demand and communicate with RPE is unclear. By profiling metabolite uptake and release and analyzing metabolic genes, we have found cone-rich retinas and human macula share specific metabolic features with upregulated pathways in pyruvate metabolism, mitochondrial TCA cycle and lipid synthesis. Human neural retina and RPE have distinct but complementary metabolic features. Retinal metabolism centers on NADH production and neurotransmitter biosynthesis. The retina needs aspartate to sustain its aerobic glycolysis and mitochondrial metabolism. RPE metabolism is directed toward NADPH production and biosynthesis of acetyl-rich metabolites, serine and others. RPE consumes multiple nutrients, including proline, to produce metabolites for the retina.

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Bo Li, Ting Zhang, Wei Liu, Yekai Wang, Rong Xu, Shaoxue Zeng, Rui Zhang, Mark C Gillies, Ling Zhu, Jianhai Du. 2020-07-10. Metabolic features of mouse and human retinas: rods vs. cones, macula vs. periphery, retina vs. RPE. https://doi.org/10.1101/2020.07.10.196295

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