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bioRxiv · 10.1101/2020.07.07.192203

A rapidly adaptable biomaterial vaccine for SARS-CoV-2

Langellotto, F.·Seiler, B. T.·Yu, J.·Cartwright, M. J.·White, D.·Yeager, C.·Super, M.·Doherty, E. J.·Barouch, D. H.·Mooney, D. J.
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Abstract

ABSTRACTThe global COVID-19 pandemic motivates accelerated research to develop safe and efficacious vaccines. To address this need, we leveraged a biomaterial vaccine technology that consists of mesoporous silica rods (MSRs) that provide a sustained release of granulocyte-macrophage colony-stimulating factor (GM-CSF) and adjuvants to concentrate and mature antigen-presenting cells at the vaccine site. Here we explored the humoral responses resulting from the use of monophosphoryl lipid A (MPLA) as the adjuvant and SARS-CoV-2 spike proteins S1, S2, the nucleocapsid (N) protein, and receptor binding domain (RBD) as the target antigens. The dose of antigen and impact of pre-manufacturing of vaccines as versus loading antigen just-in-time was explored in these studies. Single shot MSR vaccines induced rapid and robust antibody titers to the presented antigens, even without the use of a boost, and sera from vaccinated animals demonstrated neutralizing activity against a SARS-CoV-2 pseudovirus. Overall, these results suggest the MSR vaccine system may provide potent protective immunity when utilized to present SARS-CoV-2 antigens.Competing Interest StatementThe authors have declared no competing interest.View Full Text

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Langellotto, F., Seiler, B. T., Yu, J., Cartwright, M. J., White, D., Yeager, C., Super, M., Doherty, E. J., Barouch, D. H., Mooney, D. J.. 2020-07-07. A rapidly adaptable biomaterial vaccine for SARS-CoV-2. https://doi.org/10.1101/2020.07.07.192203

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