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bioRxiv · 10.1101/2020.06.16.154831

Quantum dot probes for the quantitative study of drug transport by the MacAB TolC efflux pump in lipid scaffolds

Souabni, H.·Batista dos Santos, W.·Cece, Q.·Puvanendran, D.·Picard, M.
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Abstract

ABC tripartite efflux pumps are macromolecular membrane protein machineries that expel a large variety of drugs and export virulence factors from Gram negative bacteria. Using a lipid scaffold mimicking the two-membrane environment of the transporter and designing spectroscopic conditions allowing the monitoring of both ATP hydrolysis and substrate transport in real time, we show that MacAB-TolC accommodates transport and energy consumption with high coupling efficiency.

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Souabni, H., Batista dos Santos, W., Cece, Q., Puvanendran, D., Picard, M.. 2020-06-17. Quantum dot probes for the quantitative study of drug transport by the MacAB TolC efflux pump in lipid scaffolds. https://doi.org/10.1101/2020.06.16.154831

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