Bridging scales in scattering tissues via multifocal two-photon microscopy

Chen, D.; Segovia-Miranda, F.; Walker, N.; Valenzuela, J. I.; Zerial, M.; Myers, E. W.

doi:10.1101/2020.06.11.146704

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bioRxiv · 10.1101/2020.06.11.146704

Bridging scales in scattering tissues via multifocal two-photon microscopy

Chen, D.·Segovia-Miranda, F.·Walker, N.·Valenzuela, J. I.·Zerial, M.·Myers, E. W.

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Abstract

Imaging biological systems at subcellular resolution and across scales is essential to under-standing how cells form tissues, organs, and organisms. However, existing large-scale optical techniques often require harsh tissue-clearing methods that cause significant morphological changes, compromise the integrity of cell membranes, and reduce the signal of fluorescent proteins. Here, we demonstrate multifocal two-photon microscopy that enables imaging mesoscopic scattering samples in their native tissue environment at high resolution and high speed.

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Chen, D., Segovia-Miranda, F., Walker, N., Valenzuela, J. I., Zerial, M., Myers, E. W.. 2020-06-12. Bridging scales in scattering tissues via multifocal two-photon microscopy. https://doi.org/10.1101/2020.06.11.146704

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