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bioRxiv · 10.1101/2020.05.30.125286

Rrp1 translocase and ubiquitin ligase activities restrict the genome destabilising effects of Rad51 in fission yeast

Muraszko, J.·Argunhan, B.·Ito, K.·Baranowska, G.·Barg-Wojas, A.·Kramarz, K.·Kurokawa, Y.·Iwasaki, H.·Dziadkowiec, D.
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Abstract

Rad51 is the key protein in homologous recombination DNA repair and has important roles during DNA replication. Auxiliary factors regulate Rad51 activity to facilitate productive, and prevent inappropriate, recombination that could lead to genome instability. Previous genetic analyses identified a function for Rrp1 (a member of the Rad5/16-like group of SWI2/SNF2 translocases) in counteracting Rad51 activity, shared with the Rad51 mediator Swi5-Sfr1 and the Srs2 anti-recombinase. Here, we show that Rrp1 overproduction alleviates the toxicity associated with excessive Rad51 activity in a manner dependent on Rrp1 ATPase domain. Purified Rrp1 binds to DNA and has a DNA-dependent ATPase activity. Importantly, Rrp1 directly interacts with Rad51 and removes it from double-stranded DNA, confirming that Rrp1 is a translocase capable of modulating Rad51 activity. Additionally, we demonstrate that Rrp1 possesses E3 ubiquitin ligase activity with Rad51 as a substrate, suggesting that Rrp1 regulates Rad51 in a multi-tiered fashion.

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Muraszko, J., Argunhan, B., Ito, K., Baranowska, G., Barg-Wojas, A., Kramarz, K., Kurokawa, Y., Iwasaki, H., Dziadkowiec, D.. 2020-05-31. Rrp1 translocase and ubiquitin ligase activities restrict the genome destabilising effects of Rad51 in fission yeast. https://doi.org/10.1101/2020.05.30.125286

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