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bioRxiv · 10.1101/2020.05.22.108928

Protocol for analysis of glycoproteomics LC-MS data using GlycReSoft

Klein, J. A.·Zaia, J.
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Abstract

Summary/AbstractThe GlycReSoft software tool allows users to process glycoproteomics LC-MS data sets. The tool accepts proteomics database search results or a user-defined list of proteins in the sample. GlycReSoft processes LC-MS data to yield deconvoluted exact mass values. The user has the option to import a list of theoretical glycans from an external database, a curated glycan list, or a measured glycome. The tool assembles a list of theoretical glycopeptides from the lists of theoretical glycans and proteins, respectively. The program then scores the tandem mass spectra in the LC-MS data files and provides graphical views of the identified glycopeptides for each protein in the sample, and the set of glycoforms identified for each peptide sequence.

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Klein, J. A., Zaia, J.. 2020-05-26. Protocol for analysis of glycoproteomics LC-MS data using GlycReSoft. https://doi.org/10.1101/2020.05.22.108928

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