Altered Capicua expression drives regional Purkinje neuron vulnerability through ion channel gene dysregulation in Spinocerebellar ataxia type 1

Chopra, R.; Bushart, D.; Cooper, J.; Yellajoshyula, D.; Morrison, L.; Huang, H.; Scoles, D.; Pulst, S. M.; Orr, H.; Shakkottai, V.

doi:10.1101/2020.05.21.104976

bioRxiv · 10.1101/2020.05.21.104976

Altered Capicua expression drives regional Purkinje neuron vulnerability through ion channel gene dysregulation in Spinocerebellar ataxia type 1

Chopra, R.·Bushart, D.·Cooper, J.·Yellajoshyula, D.·Morrison, L.·Huang, H.·Scoles, D.·Pulst, S. M.·Orr, H.·Shakkottai, V.

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Abstract

Selective neuronal vulnerability in neurodegenerative disease is poorly understood. Using the ATXN1[82Q] model of spinocerebellar ataxia type 1 (SCA1), we explored the hypothesis that regional differences in Purkinje neuron degeneration could provide novel insights into selective vulnerability. ATXN1[82Q] Purkinje neurons from the anterior cerebellum were found to degenerate earlier than those from the nodular zone, and this early degeneration was associated with selective dysregulation of ion channel transcripts and altered Purkinje neuron spiking. Efforts to understand the basis for selective dysregulation of channel transcripts revealed modestly increased expression of the ATXN1 corepressor Capicua (Cic) in anterior cerebellar Purkinje neurons. Importantly, lentiviral overexpression of Cic in the nodular zone accelerated both aberrant Purkinje neuron spiking and neurodegeneration. These findings reinforce the central role for Cic in SCA1 cerebellar pathophysiology and suggest that only modest reductions in Cic are needed to have profound therapeutic impact in SCA1.

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Chopra, R., Bushart, D., Cooper, J., Yellajoshyula, D., Morrison, L., Huang, H., Scoles, D., Pulst, S. M., Orr, H., Shakkottai, V.. 2020-05-22. Altered Capicua expression drives regional Purkinje neuron vulnerability through ion channel gene dysregulation in Spinocerebellar ataxia type 1. https://doi.org/10.1101/2020.05.21.104976

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