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bioRxiv · 10.1101/2020.05.18.102327

O-Pair Search with MetaMorpheus for O-glycopeptide Characterization

Lu, L.·Riley, N. M.·Shortreed, M. R.·Bertozzi, C. R.·Smith, L. M.
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Abstract

We report O-Pair Search, a new approach to identify O-glycopeptides and localize O-glycosites. Using paired collision- and electron-based dissociation spectra, O-Pair Search identifies O-glycopeptides using an ion-indexed open modification search and localizes O-glycosites using graph theory and probability-based localization. O-Pair Search reduces search times more than 2,000-fold compared to current O-glycopeptide processing software, while defining O-glycosite localization confidence levels and generating more O-glycopeptide identifications. O-Pair Search is freely available: https://github.com/smith-chem-wisc/MetaMorpheus.

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Lu, L., Riley, N. M., Shortreed, M. R., Bertozzi, C. R., Smith, L. M.. 2020-05-20. O-Pair Search with MetaMorpheus for O-glycopeptide Characterization. https://doi.org/10.1101/2020.05.18.102327

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